Rat IgG ELISA (96 Determinations)

Designed for the measurement of rat IgG in rat serum, plasma, saliva, mucosa, cell culture fluid from rat cells, or other biological sample.

IMMUNO-TEK™ Rat IgG ELISA Kit features microplate wells coated with polyclonal antibodies to rat IgG to form the capture phase of the assay. Captured rat IgG reacts with detector antibody, a polyclonal anti-rat IgG conjugated with horseradish peroxidase. This antibody reacts only to the Fc portion of the immunoglobulin molecule allowing for the specific detection of IgG. Once the chromogenic substrate tetramethyl benzidine (TMB) is added, a blue color develops in proportion to the amount of rat IgG that has been bound to the antibody-coated plate. Addition of stop solution results in a color change to yellow which can be measured spectrophotometrically at 450 nm. The concentrations of rat IgG are then calculated from a standard curve.

What's included: One 96-well microplate, 12 mL bottle of detector antibody, 7 mL bottle of rat IgG standard, 100 mL bottle of assay diluent, 125 mL bottle of 10x plate wash buffer, 12 mL bottle of substrate, 12 mL bottle of stop solution, 10 microplate sealers, and a plastic storage bag. Supplier Certificate of Analysis (CoA) is included with product shipment.

• Ready-to-use reagents
• Takes less than two hours to perform
• Specifically balanced to react uniformly with all subclasses of rat IgG

• ENGLISH (PI)
• ENGLISH (SDS)